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grna gecko v2 0 plasmid libraries  (Addgene inc)
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Addgene inc grna gecko v2 0 plasmid libraries
Tmem127 and Wwp2 Are Important for Function of SteD in Dendritic Cells (A) Quantification of total MHCII surface levels in scrambled (scr) <t>gRNA,</t> Tmem127 −/− or Wwp2 −/− MutuDCs, infected with the indicated Salmonella strains. Cells were analysed by flow cytometry 20 h p.i., and amounts of surface MHCII in infected cells are expressed as a percentage of uninfected cells in the same sample. Data are from 3 independent experiments and show means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). (B) Representative FACS histograms (left) showing OVA-dependent T cell proliferation measured by CFSE dilution in different conditions after incubation with scrambled (scr) gRNA, Tmem127 −/− or Wwp2 −/− MutuDCs infected with the indicated Salmonella strains and exposed to OVA peptide. Quantification of percentage proliferating T cells for each condition (right) representing 3 independent experiments and showing means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). See also <xref ref-type=Figure S2 . " width="250" height="auto" />
Grna Gecko V2 0 Plasmid Libraries, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tmem127 and Wwp2 Are Important for Function of SteD in Dendritic Cells (A) Quantification of total MHCII surface levels in scrambled (scr) gRNA, Tmem127 −/− or Wwp2 −/− MutuDCs, infected with the indicated Salmonella strains. Cells were analysed by flow cytometry 20 h p.i., and amounts of surface MHCII in infected cells are expressed as a percentage of uninfected cells in the same sample. Data are from 3 independent experiments and show means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). (B) Representative FACS histograms (left) showing OVA-dependent T cell proliferation measured by CFSE dilution in different conditions after incubation with scrambled (scr) gRNA, Tmem127 −/− or Wwp2 −/− MutuDCs infected with the indicated Salmonella strains and exposed to OVA peptide. Quantification of percentage proliferating T cells for each condition (right) representing 3 independent experiments and showing means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). See also <xref ref-type=Figure S2 . " width="100%" height="100%">

Journal: Cell Host & Microbe

Article Title: The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules

doi: 10.1016/j.chom.2020.04.024

Figure Lengend Snippet: Tmem127 and Wwp2 Are Important for Function of SteD in Dendritic Cells (A) Quantification of total MHCII surface levels in scrambled (scr) gRNA, Tmem127 −/− or Wwp2 −/− MutuDCs, infected with the indicated Salmonella strains. Cells were analysed by flow cytometry 20 h p.i., and amounts of surface MHCII in infected cells are expressed as a percentage of uninfected cells in the same sample. Data are from 3 independent experiments and show means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). (B) Representative FACS histograms (left) showing OVA-dependent T cell proliferation measured by CFSE dilution in different conditions after incubation with scrambled (scr) gRNA, Tmem127 −/− or Wwp2 −/− MutuDCs infected with the indicated Salmonella strains and exposed to OVA peptide. Quantification of percentage proliferating T cells for each condition (right) representing 3 independent experiments and showing means ± SD. ∗∗∗ p < 0.001, ns, not significant (two-way ANOVA followed by Tukey’s multiple comparison test). See also Figure S2 .

Article Snippet: The gRNA GeCKO v2.0 plasmid libraries (A and B pooled) (Addgene #1000000049, generated by the Feng Zhang laboratory) was packaged in lentivirus by co-transfection in HEK293ET cells with pMD2.G and psPAX2 ( ).

Techniques: Infection, Flow Cytometry, Comparison, Incubation